MOUSE Anti-LIPOPROTEIN LIPASE:Biotin Antibody

475 EUR

100ug

GEN224627

5D2

N/A

IgG1

anticorps

Antibodies

Monoclonal

Mus musculus

Biotinylated

LPL; LPL; N/A; LPL

LPL; LPL; N/A; LPL

LPL; LPL; N/A; LPL

LIPOPROTEIN LIPASE

Mouse (Mus musculus)

Mnoclonal antibodies

IgG concentration 0.1 miligram per 1 mililiter

Biotin (Purified IgG conjugated to Biotin - liquid)

ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

lipoprotein lipase; Lipoprotein lipase; lipoprotein lipase; lipoprotein lipase

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Bovine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

LIPOPROTEIN LIPASE This item specifically recognises an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. _x000D__x000D_A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. _x000D__x000D_Clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Clone 5D2 has been shown to inhibit the activity of human LPL(2,3), and is widely used in human studies.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.