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- HAMSTER ANTI MOUSE CD27:Biotin Antibody
Product short description
Price:
288 EUR
Size:
100ug
Catalog no.:
GEN224636
Product detailed description
Gene name synonims
N/A
Immunoglobulin isotype
IgG
Purification method
N/A
Also known as
CD27
Gene name
CD27
Clone
LG.3A10
Host organism
Hamster
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Conjugation
Biotinylated
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Concentration
IgG concentration 500ug/1000ul
Other gene names
Cd27; Cd27; Tnfrsf7; MGC112688
Form/Appearance
Biotin (Purified IgG conjugated to Biotin - liquid)
Tested applications:
Immunoprecipitation (IP), Immunohistology - Frozen (IHC), Flow Cytometry (FACS)
Other names
CD27 antigen; CD27 molecule; CD27 antigen; CD antigen 27; tumor necrosis factor receptor superfamily, member 7; CD27 molecule; N/A
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Storage and shipping
Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE. the antibody should be stored undiluted. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Specificity and cross-reactivity
CD27 Clone LG.3A10 recognises mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a costimulatory signal in lymphocyte proliferation and immunoglobulin production. CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein). ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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