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- MOUSE Anti-RAT CD1d:Biotin Antibody
Product short description
Price:
359 EUR
Size:
100ug
Catalog no.:
GEN224703
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Also known as
CD1d
Gene name
CD1d
Clone
WTH1
Immunoglobulin isotype
IgG2a
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Concentration
IgG concentration 500ug/1000ul
Latin name
Mus musculus,Rattus norvegicus
Form/Appearance
Biotin (Purified IgG conjugated to Biotin - liquid)
Other gene names
Cd1d1; Cd1d1; Cd1a; Cd1d; CD1.1; Ly-38; AI747460; Cd1.1
Tested applications:
Functional Assay (FA), Flow Cytometry (FACS), Immunohistology - Frozen (IHC), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Other names
antigen-presenting glycoprotein CD1d1; Antigen-presenting glycoprotein CD1d1; antigen-presenting glycoprotein CD1d1; T-cell surface glycoprotein CD1d1; CD1d1 antigen; N/A
Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Specificity and cross-reactivity
CD1d This item recognises rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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