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- HAMSTER ANTI MOUSE NOTCH 2:RPE Antibody
Product short description
Price:
310 EUR
Size:
100 Tests
Catalog no.:
GEN224718
Product detailed description
Gene name synonims
N/A
Immunoglobulin isotype
IgG
Purification method
N/A
Concentration
N/A
Also known as
NOTCH 2
Gene name
NOTCH 2
Clone
HMN2-35
Host organism
Hamster
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Other gene names
Notch2; Notch2; N2; AI853703; Notch 2
Form/Appearance
RPE (Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised)
Other names
neurogenic locus notch homolog protein 2; Neurogenic locus notch homolog protein 2; neurogenic locus notch homolog protein 2; Motch B; Notch gene homolog 2; notch 2; Motch B
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. After reconstitution Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 12 months from date of reconstitution.
Specificity and cross-reactivity
NOTCH 2 Clone HMN2-35 specifically recognises Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. _x000D__x000D_The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. _x000D__x000D_Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. _x000D__x000D_Signalling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukaemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. _x000D__x000D_Clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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