Rat Monoclonal [clone 12] (IgG2a) to Bovine Myelin Basic Protein / MBP Antibody

597 EUR

0.125 ml

GEN245954

12

MBP

N/A

N/A

N/A

IgG2a

anticorps

Antibodies

Monoclonal

Rattus norvegicus

MBP; MBP; N/A; MBP

Mnoclonal antibodies

Rat (Rattus norvegicus)

Myelin Basic Protein / MBP

Tissue culture supernatant, 0.1M Tris, 0.1% sodium azide

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA), Radioimmunoassay (RIA)

Basic proteins pH>7 are often produced recombinant and shouldn’t be precipitated by formic acid buffer.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

myelin basic protein isoform 2; Myelin basic protein; Golli-MBP; myelin basic protein; myelin A1 protein; myelin membrane encephalitogenic protein; myelin basic protein; Myelin A1 protein; Myelin membrane encephalitogenic protein

For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.

Bovine, Chicken, Guinea pig, Human (Homo sapiens), Mammal, Mouse (Mus musculus), Pig, Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus), Sheep; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Reacts with myelin basic protein from a wide range of species. The Reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide Reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.