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- Mouse Monoclonal [clone DE-K13] (IgG2a) to Human Cytokeratin 10+13 Antibody
Product short description
Price:
597 EUR
Size:
50ug
Catalog no.:
GEN246064
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Immunoglobulin isotype
IgG2a
Clone
DE-K13
Purification method
Purified
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Also known as
Cytokeratin 10+13
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Concentration
1 miligram per 1 mililiter
Form/Appearance
PBS containing 0.09% sodium azide
Tested applications:
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunocytochemistry (ICC), Western Blot (WB), Flow Cytometry (FC/FACS)
Species reactivity
Cat, Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
Store at +4 degrees Celsius for short term storage. For long term storage aliquot and keep the antibody at -20 degrees Celsius. Freeze-thaw cycles should be avoided in order to retain the activity of the antibody. Store undiluted.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Specificity and cross-reactivity
Reacts with cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeratin 13 in formalin-fixed, paraffin-embedded tissues. Cytokeratin 10 is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while cytokeratin 13 is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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