Mouse Monoclonal [clone AE3] (IgG1) to Human Cytokeratin Type II Antibody

597 EUR

50ug

GEN246262

AE3

IgG1

anticorps

Antibodies

Monoclonal

Mus musculus

Purified IgG

Cytokeratin Type II

Mouse (Mus musculus)

Mnoclonal antibodies

1 miligram per 1 mililiter

N/A; KRT75; N/A; K6HF; KB18; CK-75; hK6hf; K75

N/A; KRT75; N/A; K6HF; KB18; CK-75; hK6hf; K75

N/A; KRT75; N/A; K6HF; KB18; CK-75; hK6hf; K75

0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)

Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable.

cytokeratin type II; Keratin, type II cytoskeletal 75; N/A; N/A; Cytokeratin-75; CK-75; Keratin-6 hair follicle; hK6hf; Keratin-75; K75; Type II keratin-K6hf; Type-II keratin Kb18

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.