Rat Monoclonal [clone YL1/2] (IgG2a) to Yeast Alpha Tubulin Antibody

597 EUR

50ug

GEN246406

N/A

N/A

N/A

N/A

IgG2a

YL1/2

anticorps

Antibodies

Monoclonal

Alpha Tubulin

Rattus norvegicus

Affinity Purified

Mnoclonal antibodies

Rat (Rattus norvegicus)

1 miligram per 1 mililiter

PBS, pH 7.4, 0.09% sodium azide.

The Monoclonal [clone YL1/2] (IgG2a) Yeast Alpha Tubulin Antibody is a α- or alpha protein sometimes glycoprotein present in blood.

Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Radioimmunoassay (RIA)

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Echinoderm, Avian, Human (Homo sapiens), Mammal, Yeast; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin). The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. This product is routinely tested in ELISA on Tubulin; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.