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- MBS Monoclonals
- Mouse Monoclonal (IgG2a,k) to Human ACTA2 / Smooth Muscle Actin Antibody
Product short description
Price:
597 EUR
Size:
0.05 ml
Catalog no.:
GEN246689
Product detailed description
Gene name synonims
N/A
Concentration
N/A
Clone
N/A
Gene name
ACTA2
Tissue
muscle
Immunoglobulin isotype
IgG2a,k
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Purification method
Tissue culture supernatant
Also known as
ACTA2 / Smooth Muscle Actin
Other gene names
ACTA2; ACTA2; AAT6; ACTSA; MYMY5; ACTSA; ACTVS
Form/Appearance
20 mM Tris-borate, 150 mM sodium chloride, 0.09% sodium azide.
Tested applications:
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
Other names
actin, aortic smooth muscle; Actin, aortic smooth muscle; actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein; actin, alpha 2, smooth muscle, aorta; Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Storage and shipping
For long term storage, keep the antibody at -20 degrees Celsius. Short term storage of the antibody can be done at +4 degrees Celsius. Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity.
Species reactivity
Human (Homo sapiens), Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Specificity and cross-reactivity
This antibody reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fibroblasts, striated muscle and myocardium. This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. Species cross-reactivity: Cross-reacts with Human, Rabbit, Rat, porcine, and Baboon.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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