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- MBS Monoclonals
- Mouse Monoclonal [clone 4C2] (IgG1) to Human ACTB / Beta Actin Antibody
Product short description
Price:
597 EUR
Size:
50ug
Catalog no.:
GEN249041
Product detailed description
Gene name synonims
N/A
Clone
4C2
Gene name
ACTB
Immunoglobulin isotype
IgG1
Purification method
Purified
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Also known as
ACTB / Beta Actin
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Form/Appearance
PBS, 0.09% sodium azide
Concentration
1 miligram per 1 mililiter
Other gene names
ACTB; ACTB; BRWS1; PS1TP5BP1; N/A
Tested applications:
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunofluorescence (IF), Western Blot (WB)
Other names
actin, cytoplasmic 1; Actin, cytoplasmic 1; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; Beta-actinActin, cytoplasmic 1, N-terminally processed
Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Pig, Rabbit (Oryctolagus cuniculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Specificity and cross-reactivity
Mouse anti Human actin beta, clone 4C2, recognizes cytoplasmic actin beta (known also as ACTB and Beta Actin), a 41.7 kD cytoskeletal protein. Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Six different isoforms of actin have been identified in mammals. Clone 4C2 is highly specific for cytoplasmic actin beta and does not react with other actin isoforms. Mouse anti Human actin beta, clone 4C2 has been used to look at the remodeling of the actin cytoskeleton following Sendai virus infection and the specific role of the viral M protein in actin remodeling.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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