Mouse Monoclonal [clone N-GAD65] (IgG1) to Human GAD65 Antibody

597 EUR

50ug

GEN249171

GAD2

IgG1

GAD65

GAD65

N-GAD65

anticorps

Antibodies

Monoclonal

Mus musculus

Protein G Purified

Mnoclonal antibodies

Mouse (Mus musculus)

PBS, 0.09% sodium azide

1 miligram per 1 mililiter

GAD2; GAD2; GAD65; GAD65; GAD-65

Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Human (Homo sapiens), Monkey, Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Specifically recognizes an epitope within the N-terminal (NT) region of Glutamate decarboxylase 2, otherwise known as GAD65/GAD2, an amphiphilic membrane-anchored protein and member of the group 2 decarboxylase family, principally expressed in the brain and also in pancreatic beta cells. GAD65 catalyzes the decarboxylation of glutamate to GABA, the major inhibitory neurotransmitter in the central nervous system. GAD65 is the 65kD isoform of GAD, encoded by the GAD2 gene, which is predominantly expressed by nerve termini, as oppose to the 67kD isoform (GAD67), which is predominantly found in the cell body, and is encoded by the GAD1 gene. Studies have shown the presence of autoantibodies to GAD65 (GAD65Ab) in autoimmune diseases, including Graves disease and Stiff Man Syndrome (SMS), but GAD65Ab are most prevalent in patients with Type I diabetes mellitus, and those at high risk of developing Type I diabetes. The N-Terminal region of GAD65 lacks the epitopes for GAD65Ab in Type I diabetes patients, and has been shown as essential for targeting the enzyme to GABA-containing secretory vesicles. Clone N-GAD65 has been shown to be highly specific for GAD65 and does not recognize GAD67.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.