Product short description
Price:
354 EUR
Size:
100ug
Catalog no.:
GEN438174
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Form/Appearance
N/A
Purification method
N/A
Other names
N/A
Immunoglobulin isotype
IgG1
Also known as
Biotin
Clone
SPM375
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Tested applications:
Confocal Microscopy, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), In situ Nucleic Acid Hybridization, Western Blot (WB), Immunohistology (IH)
Species reactivity
All species; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
Store the antibody at +4C. Temperature variations in the range of 1 to 7 degrees Celsius are tolerable. We recommend NOT to freeze the antibody. Antibody is stable for 24 months.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Specificity and cross-reactivity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.