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- MBS Monoclonals
- MHC Class 1, H2, Db, alpha3 (Biotin) Antibody
Product short description
Price:
553 EUR
Size:
500ug
Catalog no.:
GEN602309
Product detailed description
Gene name
N/A
Concentration
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Clone
5K48
Immunoglobulin isotype
IgG2a,k
Purification method
Purified
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Host organism
Mouse (Mus musculus)
Also known as
MHC Class 1, H2, Db, alpha3
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Form/Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes the alpha3 domain of H-2Db class I MHC antigen. Crossreacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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