MHC Class 2, I-A (Biotin) Antibody

498 EUR

100ug

GEN632523

N/A

IgG1

3H437

anticorps

Antibodies

Monoclonal

Biotinylated

Purified IgG.

MHC Class 2, I-A

Mouse (Mus musculus)

Mnoclonal antibodies

orla-uaa; N/A; N/A; N/A

orla-uaa; N/A; N/A; N/A

orla-uaa; N/A; N/A; N/A

Rat (Rattus norvegicus)

Flow Cytometry (FC/FACS)

Store the antibody at -20 degrees Celsius.

Mhc class I A; N/A; Mhc class I A; N/A; N/A

Supplied as a liquid, 1% BSA, 0.09% sodium azide.

Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Recognizes a polymorphic determinant of rat Ia antigen present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains (1,2). The antibody crossreacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognizes la antigens on B-cells, dendritic cells and certain epithelial cells (1, 3, 4). It is particularly useful for distinguishing la positive cells from different rat strains, eg for recognizing cells of donor origin in bone marrow reconstituted radiation chimeras (3,4).This clone has been reported to be suitable for immunohistochemical of frozen sections (6.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.