Product short description
Price:
619 EUR
Size:
500ug
Catalog no.:
GEN603185
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Other names
N/A
Clone
5K115
Also known as
RLA, DQ
Immunoglobulin isotype
IgG2a,k
Purification method
Purified
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Conjugation
Biotinylated
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Source organism
Rabbit (Oryctolagus cuniculus)
Form/Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Tested applications:
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Species reactivity
Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity and cross-reactivity
Recognizes rabbit RLA-DQ alpha chain (Mr ~35kD). Reacts with RLA-DQ-transfected cells, but not with RLA-DR-transfected cells. In normal adult rabbits, the antibody reacts with 30-60% of peripheral blood leukocytes. It has also been reported that the antibody stains: lymphocytes and macrophages in the spleen; lymphocytes in the thymic medulla and some cells in the cortex; Kupffer cells in the liver; and synovial lining cells (i.e., infiltrating macrophages) in inflamed synovium.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.