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- Mouse Anti-Histone H4 (mono methyl K20) [5E10-D8]
Product short description
Price:
514 EUR
Size:
100ul
Catalog no.:
GEN375123
Product detailed description
Immunoglobulin isotype
IgG1
Clone
5E10-D8
Purification method
Protein G
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Concentration
1 miligram per 1 mililiter
Also known as
Histone H4 (mono methyl K20)
Other gene names
HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
Gene name synonims
HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
Gene name
HIST2H4B; HIST1H4A; H4/o; H4/A; H4FA
Form/Appearance
Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Other names
histone H4; Histone H4; histone H4; histone 2, H4b; histone cluster 2, H4b; N/A
Tested applications:
Dot Blot (DB), Western Blot (WB) against endogenous protein, ELISA (EIA), Chromatin Immunoprecipitation (ChIP)
Species reactivity
Human (Homo sapiens). Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Specific for K20 mono-methylated histone H4; no cross-reactivity with K20 tri-, di-, or unmethylated histone H4; no cross-reactivity with histone H3.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
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