Mouse Anti-Nestin [2C1.3A11]- Neural Stem Cell Marker

514 EUR

100ul

GEN375191

IgG1

Nestin

2C1.3A11

Protein G

Antibodies

Monoclonal

cell, stem

Mus musculus

Mouse (Mus musculus)

Mnoclonal antibodies

NES; NES; Nbla00170; N/A

NES; NES; Nbla00170; N/A

NES; NES; Nbla00170; N/A

1 miligram per 1 mililiter

nestin; Nestin; nestin; nestin; N/A

Purified antibody containing PBS 0.1% sodium azide;

Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP)

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.For cells, cell lines and tissues in culture till half confluency.

Lyophilized antibodies may be transported at ambient temperature and stored for short terms at +4 degrees Celsius and at -20 for long term storage. Antibodies in liquid form can be shipped and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol has to added and the vial must be stored at -20°C.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.