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- MBS Monoclonals
- Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti-FLAG)
Product short description
Price:
199 EUR
Size:
0.01 mililiter
Catalog no.:
GEN435026
Product detailed description
Host organism
N/A
Tested applications:
N/A
Gene name
N/A
Concentration
N/A
Form/Appearance
N/A
Purification method
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Clone
N/A
Category
Antibodies
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IgG2b
Conjugation
Agarose,Flag
Also known as
Anti-DYKDDDDK-Tag
Subcategory
Mnoclonal antibodies
Species reactivity
All; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Storage and shipping
The product is supplied as a 50% slurry in storage buffer (1x PBS, pH 7.4, containing 0.1% NaN3). Store the product at Store the antibody at +4 degrees Celsius. and do not freeze.
Specificity and cross-reactivity
Anti-DYKDDDDK-Tag Mouse mAb detects transfected proteins containing the DYKDDDDK epitope tag.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
The purest agarose was used in the production of Anti-DYKDDDDK-Tag mAb (Agarose conjugated) (Same as Sigma's Anti- ) by MBS Monoclonals.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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