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- Anti-Mouse CD4 Biotin (Clone YTS 191.1) (rat IgG2b)
Product short description
Price:
376 EUR
Size:
0.3 miligrams
Catalog no.:
GEN520106
Product detailed description
Also known as
CD4
Gene name
CD4
Gene name synonims
N/A
Purification method
N/A
Form/Appearance
Biotin
Immunoglobulin isotype
Rat IgG2b
Category
Antibodies
Clonality
Monoclonal
Clone
YTS 191.1.2
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Rat (Rattus norvegicus)
Concentration
0.1 miligram per 1 mililiter
Latin name
Mus musculus,Rattus norvegicus
Other gene names
CD4; CD4; CD4mut; CD4 receptor
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Other names
CD4; CD4 protein; T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD4 molecule; N/A
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
CD4 (L3/T4; Ly4) (Mouse CD4 (L3/T4)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Storage and shipping
For all formats, store the antibody at +4 degrees Celsius. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin, FITC, No Azide), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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