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- Anti-Rat CD45 Biotin (Clone OX-30) (mouse IgG2a)
Product short description
Price:
304 EUR
Size:
100ug
Catalog no.:
GEN520137
Product detailed description
Purification method
N/A
Gene name synonims
N/A
Also known as
CD45
Gene name
CD45
Clone
OX-30
Form/Appearance
Biotin
Category
Antibodies
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IGg2a
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Other gene names
ptprc; N/A; N/A; N/A
Host organism
Mouse (Mus musculus)
Other names
CD45; N/A; CD45; N/A; N/A
Concentration
0.1 miligram per 1 mililiter
Latin name
Mus musculus,Rattus norvegicus
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Storage and shipping
For long term storage (Purified, Biotin and FITC), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage.
Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
CD45 (LCA) (Rat CD45); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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