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- Anti-Rat CD62L Biotin (Clone OX-85) (mouse IgG1)
Product short description
Price:
304 EUR
Size:
100ug
Catalog no.:
GEN520192
Product detailed description
Gene name synonims
N/A
Other names
N/A
Purification method
N/A
Other gene names
N/A
Clone
OX-85
Gene name
CD62L
Also known as
CD62L
Form/Appearance
Biotin
Category
Antibodies
Immunoglobulin isotype
Mouse IgG1
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Latin name
Mus musculus,Rattus norvegicus
Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), ELISA (EIA)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Storage and shipping
Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. For long term storage, aliquot and freeze unused portions at - 20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Specificity and cross-reactivity
CD62L (L-selectin, LECAM) (Rat Leukocyte Common Antigen (L-selectin)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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