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- Anti-Mouse H-2KbDb (private), FITC (Clone 5041.16.1) (mouse IgG2a)
Product short description
Price:
299 EUR
Size:
100ug
Catalog no.:
GEN520324
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Purification method
N/A
Other names
N/A
Form/Appearance
FITC
Clone
5041.16.1
Category
Antibodies
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IgG2a
Latin name
Mus musculus
Also known as
H-2KbDb (private)
Conjugation
Anti-FITC Antibody
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Tested applications:
Immunohistochemistry (IHC)
Concentration
Antibody Concentration: 0.1 mg / ml
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
H-2KbDb (Mouse H-2KbDb); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze the antibody at -20 degrees Celsius. Keep the unused portions of the antibody in volumes suitable for single use. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity. Avoid prolonged exposure to light.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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