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- Anti-Mouse H-2Ld (public; H-2.m65), Biotin (Clone 30-5-7S) (mouse IgG2a)
Product short description
Price:
299 EUR
Size:
100ug
Catalog no.:
GEN520333
Product detailed description
Gene name
N/A
Tested applications:
N/A
Gene name synonims
N/A
Other gene names
N/A
Purification method
N/A
Other names
N/A
Form/Appearance
Biotin
Clone
30-5-7S
Category
Antibodies
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IgG2a
Latin name
Mus musculus
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Also known as
H-2Ld (public; H-2.m65)
Concentration
0.1 miligram per 1 mililiter
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
H-2Ld (Anti-mouse H-2Ld (public determinant H-2.65)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
For all formats, Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. DO NOT FREEZE PE conjugates. For long term storage (Purified, Biotin and FITC), aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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