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- Anti-Mouse I-Ab (public; Ia.m8), FITC (Clone 28-16-8S) (mouse IgM)
Product short description
Price:
299 EUR
Size:
100ug
Catalog no.:
GEN520349
Product detailed description
Additional isotype
IgM
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Purification method
N/A
Other names
N/A
Form/Appearance
FITC
Clone
28-16-8S
Immunoglobulin isotype
Mouse IgM
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Also known as
I-Ab (public; Ia.m8)
Host organism
Mouse (Mus musculus)
Tested applications:
Immunohistochemistry (IHC)
Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
I-Ab (Mouse MHC class II I-Ab, cross reacts with I-Ad); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles and prolonged exposure to light. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Check label for expiry date.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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