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- MBS Monoclonals
- Vimentin antibody (FITC)
Product short description
Price:
807 EUR
Size:
0.25 ml
Catalog no.:
GEN531077
Product detailed description
Concentration
N/A
Immunoglobulin isotype
IgG2a
Clone
VIM 3B4
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Also known as
Vimentin (FITC)
Other gene names
VIM; VIM; N/A; N/A
Gene name synonims
VIM; VIM; N/A; N/A
Gene name
VIM; VIM; N/A; N/A
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Purification method
Protein A affinity chromatography
Other names
vimentin; Vimentin; vimentin; vimentin; N/A
Form/Appearance
Protein A purified and supplied as a liquid
Tested applications:
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) (Formalin/Paraffin), Western Blot (WB)
Species reactivity
Amphibia, bovine, chicken, dog, Human (Homo sapiens), monkey; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Bovine vimentin; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
For shorter periods of time keep the antibody at +4 degrees Celsius. Long term storage should be done after aliquoting and at -20°C. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies, thus reducing their activity.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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