FLAG antibody

498 EUR

100ug

GEN533391

N/A

N/A

FLAG

Flag

FLAG

FG4R

IgG2b

FlaG; N/A

anticorps

Monoclonal

Antibodies

Mnoclonal antibodies

Mouse (Mus musculus)

1 miligram per 1 mililiter

Supplied as a liquid in 10mM PBS, pH 7.2, with 0.05% NaN3

FLAG antibody was purified by Protein A affinity chromatography.

Dot Blot (DB), ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

FLAG tag; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

For optimal storage conditions, keep the antibody at +4°C. Temperature variations in the range 1°C to 9°C are tolerable. for several days to weeks. Add 0.1% NaN3 and store at -20 deg C for longer periods. Avoid repeated freeze/thaw cycles.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.