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- Monoclonal mouse anti-epithelial cell adhesion molecule (EpCAM), human
Product short description
Price:
713 EUR
Size:
1000ug
Catalog no.:
GEN660277
Product detailed description
Gene name synonims
N/A
Concentration
N/A
Host organism
N/A
Tissue
cell
Gene name
EpCAM
Category
Antibodies
Clonality
Monoclonal
Clone
B6 and 4C10
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Tested applications:
ELISA (EIA), Western Blot (WB)
Also known as
Epithelial cell adhesion molecule
Immunoglobulin isotype
IgG2a for MAb B6 IgG2b for MAb 4C10
Purification method
Chromatography on protein A Sepharose
Form/Appearance
PBS, pH 7.4, 0.1 % sodium azide (NaN3)
Storage and shipping
Store the antibody at +4 degrees Celsius.
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other gene names
EPCAM; EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Ep-CAM; EGP; EGP314; hEGP314
Specificity and cross-reactivity
MAb B6: EpCAM, human a.a.r. 24-265 MAb 4C10: EpCAM, human a.a.r. 116-242; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the Monoclonal mouse anti-epithelial adhesion molecule (EpCAM).This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are present in lysates used as reference for ELISA quantification of these molecules and their subunits.
Other names
Epithelial cell adhesion molecule; Epithelial cell adhesion molecule; epithelial cell adhesion molecule; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); epithelial cell adhesion molecule; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA; Major gastrointestinal tumor-associated protein GA733-2; Tumor-associated calcium signal transducer 1; CD_antigen: CD326
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