anti-mouse CD3-Biotin

393 EUR

100ug

GEN666009

CD3

CD3

N/A

IgG

N/A

Hamster

145-2C11

1.0 mg/ml

Monoclonal

Antibodies

Mus musculus

Biotinylated

N/A; cd3g; N/A; N/A

Mnoclonal antibodies

cd3; CD3gamma/delta; N/A; N/A; CD3gamma/delta

Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA)

50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4°C. Freezing/thawing cycles should be avoided in order to retain the full activity. Do not expose the vials to direct bright light. If stored under proper conditions, the antibody will retain its activity for at least one year.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.