Mouse Anti-Cytokeratin 8-FITC

387 EUR

100ug

GEN670011

N/A

N/A

SB37b1

Antibodies

Monoclonal

Mus musculus

Cytokeratin 8

Anti-FITC Antibody

Mouse (Mus musculus)

Mouse (BALB/c) IgG2a

Mnoclonal antibodies

Fluorescein (FITC) Conjugate

ELISA; Immunohistochemistry; Western Blot

KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8

KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8

KRT8; KRT8; K8; KO; CK8; CYK8; K2C8; CARD2; CYK8

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

cytokeratin 8; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; CK-8; keratin-8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8; keratin 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2; Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.1 mg in 0.2 mL PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius. or long-term the antibody should be stored at -20 degrees Celsius.. Reagents are stable for the period shown on the label if stored as directed.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

Keratin 8 (55 kDa)1 Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1-8). The two keratin types share only 30% sequence homology.2-6 Keratins demonstrate tissue- and differentiation-specific expression profiles. Cytokeratin 8 (55 kDa) belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. It is expressed in all simple type epithelia tissues (e.g., liver, pancreas, kidney, gut epithelial lining) but not in stratified squamous epithelia. Cytokeratin 8 is also present in the majority of adenocarcinomas and ductal carcinomas but is absent in squamous cell carcinomas. The SB37b monoclonal antibody reacts specifically with cytokeratin 8 and recombinant C-terminal keratin 8.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.