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- Hamster Anti-Mouse CD3e-APC/Cy5.5
Product short description
Price:
359 EUR
Size:
100ug
Catalog no.:
GEN670248
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Purification method
N/A
Other names
N/A
Clone
500-A2
Category
Antibodies
Clonality
Monoclonal
Also known as
CD3eC/Cy5.5
Conjugation
Cy5.5,Cy5.5
Immunoglobulin isotype
IgG, epsilon
Latin name
Mus musculus
Host organism
Syrian Hamster
Subcategory
Mnoclonal antibodies
Form/Appearance
Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) Conjugate
Conjugated
These antibodies are excite for emission at 678nm and detected at a 694 nm wavelengths.
Tested applications:
Identification and enumeration of CD3+ cells by Flow Cytometry 1, Immunoprecipitation 2, In vitro activation of T cells 2, 3, 4
Storage and shipping
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
Mouse CD3epsilon, Mr 25 kDa CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR).CD3epsilon is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. 500-A2 recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex of mouse strains tested.1, 2, 3; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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