Mouse Anti-Human CD86/B7-2-FITC

370 EUR

100 Tests

GEN670289

N/A

N/A

IgG1

BU63

CD86/B7-2

Antibodies

Monoclonal

Mus musculus

Anti-FITC Antibody

Mouse (Mus musculus)

Mnoclonal antibodies

LOC554318; N/A; N/A; N/A

LOC554318; N/A; N/A; N/A

LOC554318; N/A; N/A; N/A

Fluorescein (FITC) Conjugate

Flow Cytometry, Immunoprecipitation, Immunohistochemistry (Frozen sections), Western Blot

CD86/B7-2 costimulatory molecule; N/A; CD86/B7-2 costimulatory molecule; CD86 antigen; B7-1 costimulatory molecule; N/A; N/A

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

The fluorescein (FITC) conjugate is supplied as 0.1mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Human CD86/B7-2 CD86, also known as B70/B7-2, is a 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily. It is expressed at high levels on resting monocytes and dendritic cells and at very low levels on resting B and T lymphocytes. Activation of B cells, T cells and monocytes leads to upregulation of CD86 expression. Like CD80, CD86 is a ligand of CD28 and CD152. The interactions of CD86 (and CD80) with CD28 provides important costimulatory signals to T cells activated via the CD3/TCR complex. CD86 is expressed earlier than CD80 and appears to dominate in the primary immune response.1-3; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.