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- Mouse Anti-Chicken CD8a-PE/CY5
Product short description
Price:
404 EUR
Size:
100ug
Catalog no.:
GEN670297
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Purification method
N/A
Other names
N/A
Clone
CT-8
Conjugation
Cy5.5
Category
Antibodies
Clonality
Monoclonal
Also known as
CD8a-PE/CY5
Immunoglobulin isotype
IgG1, kappa
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Form/Appearance
Spectral Red (SPRD) Conjugate
Tested applications:
Identification and enumeration of CD8+cells by Flow Cytometry 2, Identification of CD8+ T cells in acetone-fixed, Frozen tissue sections4, Immunoprecipitation 2
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze!
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
alpha chain (34-kDa) of chicken CD8 In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gammadelta CD8alphabeta cells in the chicken that is not found in rodents.1,3 Chicken CD8 is expressed on approximately 80% of thymocytes, 15% of blood mononuclear cells and 50% of spleen cells, but
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