Rat Anti-Mouse CD45R/B220-AF488

337 EUR

100ug

GEN670324

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RA3-6B2

Antibodies

Monoclonal

IgG2a, kappa

CD45R/B220-AF488

Mnoclonal antibodies

Rat (Rattus norvegicus)

Mus musculus,Rattus norvegicus

Alexa Fluor 488 (AF488) Conjugate

Flow Cytometry, Immunohistochemistry (Frozen sections)

The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

B220 isoform of CD45, Mr 240 kDa CD45R/B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and, prior to the availability of anti-CD19 MAbs, was commonly used as a pan B-cell marker.1-5 It also reacts with certain activated T cells, as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells.1, 6 In vivo administration of RA3-6B2 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice.7, 8; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.