Rat Anti-Mouse CD3e-CY5

310 EUR

100ug

GEN670329

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Cy5.5

CD3e-CY5

Monoclonal

Antibodies

IgG2b, kappa

C363.29B (YCD3-1)

Mnoclonal antibodies

Rat (Rattus norvegicus)

Cyanine 5 (CY 5) Conjugate

Mus musculus,Rattus norvegicus

Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.

The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Identification and enumeration of CD3+ cells by Flow Cytometry 1, 2, Immunoprecipitation 1, In vitro depletion of CD3+ cells 1, In vitro activation of T cells (accessory cells must be provided) 1, 3, 4 Immunohistochemistry (PS)5

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mouse CD3epsilon (Mr 25 kD) CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains.1, 2 CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.1 In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naïve T cell in vitro. Immobilized C363.29 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present.1, 3, 4 The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody.1; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.