Mouse Anti-Human CD34-FITC

382 EUR

100 Tests

GEN670357

N/A

N/A

CD34

IgG1

Antibodies

Monoclonal

Mus musculus

581 (Class III)

Anti-FITC Antibody

Mouse (Mus musculus)

CD34; CD34; N/A; N/A

CD34; CD34; N/A; N/A

CD34; CD34; N/A; N/A

Mnoclonal antibodies

Fluorescein (FITC) Conjugate

Flow Cytometry, Immunohistochemistry (Frozen sections)

CD34; Hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell antigen CD34; CD34 antigen; CD34 molecule; N/A

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

The fluorescein (FITC) conjugate is supplied as 100 tests in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius..

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Human CD34 Class III epitope CD34 is a 110-120 kDa heavily O-glycosylated type I transmembrane lycoprotein. It is expressed on a small subpopulation (2-4%) of bone marrow cells including hematopoietic stem cells, small vessel endothelial cells and tumors of epithelial origin, and a subset of fibroblasts. It is believed that hematopoietc CD34 plays a role in modulating adhesion and as well as signal tranduction through its phosphorylation by activated protein kinase C. The monoclonal antibody 581 reacts to the class III CD34 epitope which is resistant to euraminidase, chymopapain and glycoprotease.1-8; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.