Rat Anti-Mouse CD2/LFA-2-FITC

431 EUR

500ug

GEN673122

N/A

N/A

42353

Monoclonal

CD2 (LFA-2)

Rat IgG1 kappa

Anti-FITC Antibody

Secondary Antibodies

Mnoclonal antibodies

Rat (Rattus norvegicus)

Fluorescein (FITC) Conjugate

Mus musculus,Rattus norvegicus

Cd2; Cd2; Ly37; LFA-2; Ly-37; Ly-37

Flow Cytometry; Immunoprecipitation

Cd2; Cd2; Ly37; LFA-2; Ly-37; Ly-37

Cd2; Cd2; Ly37; LFA-2; Ly-37; Ly-37

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

CD2 antigen; T-cell surface antigen CD2; T-cell surface antigen CD2; LFA-3 receptor; lymphocyte antigen 37; T-cell surface antigen T11/Leu-5; CD2 antigen; LFA-2; LFA-3 receptor; Lymphocyte antigen 37; Ly-37; T-cell surface antigen T11/Leu-5

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mouse CD2/LFA-2 (Mr 45-58 kDa) CD2, also known as leukocyte function-associated antigen-2 (LFA-2), is a member of the immunoglobulin superfamily of cell adhesion molecules.^1 It is expressed on the surface of >95% of thymocytes, and essentially all B cells, T cells and NK cells. ^1-3 In the mouse, CD2 is the receptor for CD48.^4 CD2 is thought to play a major role in mediating T-cell adhesion, T-cell recognition/activation, Tcell signaling and intrathymic differentiation.^5-10; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.