Rat Anti-Mouse CD18-BIOT

398 EUR

500ug

GEN673232

N/A

N/A

C71/16

Monoclonal

Secondary Antibodies

Mnoclonal antibodies

Rat (Lewis) IgG2a kappa

Biotin (BIOT) Conjugate

Rat (Rattus norvegicus)

CD18; N/A; CD18; N/A; N/A

CD18/Integrin beta 2 Chain

LOC100304487; N/A; N/A; N/A

LOC100304487; N/A; N/A; N/A

LOC100304487; N/A; N/A; N/A

Mus musculus,Rattus norvegicus

Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Common beta 2 subunit (Mr 95 kDa) of the LFA-1 integrin complex CD18 represents the common beta 2 integrin subunit that associates non-covalently with the alpha beta chains of CD11a/LFA-1a, CD11b/Mac-1, and CD11c to form various integrin heterodimers. It is expressed strongly on lymphocytes and monocytes, and weakly on granulocytes, CD18 mediates a variety of heterotypic and homotypic intercellular adhesion reactions, and it regulates the ligand-binding activities of the various CD11/CD18 complexes. MAb C71/16 has not been reported to inhibit cell adhesion.^1-4; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.