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- Rat Anti-Mouse CD45RC-BIOT
Product short description
Price:
437 EUR
Size:
500ug
Catalog no.:
GEN673400
Product detailed description
Purification method
N/A
Other names
N/A
Gene name
N/A
Other gene names
N/A
Concentration
N/A
Gene name synonims
N/A
Clone
GL24
Also known as
CD45RC
Clonality
Monoclonal
Immunoglobulin isotype
Rat IgM kappa
Category
Secondary Antibodies
Subcategory
Mnoclonal antibodies
Form/Appearance
Biotin (BIOT) Conjugate
Host organism
Rat (Rattus norvegicus)
Latin name
Mus musculus,Rattus norvegicus
Tested applications:
Flow Cytometry; Immunoprecipitation
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
An exon C-dependent epitope of CD45 glycoprotein CD45RC represents an exon C-dependent isoform of CD45 glycoprotein. It is expressed at high density on all B lymphocytes, at intermediate density on most CD8^+ splenic T cells, and on two subpopulations of CD4^+ splenic T cells with high and low density of expression. CD45RC is also present on a small subset of thymocytes.^1-4; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein-5-maleimide (FLMA) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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