Rat Anti-Mouse BP-1/Ly-51-FITC

453 EUR

500ug

GEN673491

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FG35.4

Monoclonal

Rat IgG1 kappa

BP-1 (Ly-51, 6C3)

Anti-FITC Antibody

Mnoclonal antibodies

Secondary Antibodies

Rat (Rattus norvegicus)

Fluorescein (FITC) Conjugate

Mus musculus,Rattus norvegicus

Flow Cytometry; Immunoprecipitation

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

BP-1/Ly-51/6C3 antigen, (aminopeptidase A, E.C.3.4.11.7, glutamylaminopeptidase) BP-1, also known as Ly-51/6C3 antigen, is a type II homodimeric transmembrane glycoprotein formed by two disulfide-linked chains of 140 kDa.1, 2 Originally identified on the surface of pre-B cell lymphomas1, 3 the cDNA encoding BP-1/Ly-51/6C3 has been cloned and found to represent aminopeptidase A.4, 5 It is expressed at high levels on bone marrow stromal cell lines which support in vitro B lymphopoiesis and on a wide variety of mouse tissues known to possess aminopeptidase activity.6-8 Subsets of normal bone marrow pre-B and B cells express low levels of BP-1/Ly-51/6C3, which is rapidly upregulated on pre-B cells in the presence of IL- 7.9, 10 The BP-1/6C3 marker is commonly used to identify B cells in different stages of development.11; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.