Hamster Anti-Mouse Ly-49C/F/H/I-FITC

431 EUR

500ug

GEN673543

N/A

N/A

N/A

N/A

N/A

N/A

14B11

Monoclonal

Ly49C/F/H/I

Mus musculus

Syrian Hamster

Flow Cytometry

Syrian Hamster IgG

Anti-FITC Antibody

Mnoclonal antibodies

Secondary Antibodies

Fluorescein (FITC) Conjugate

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.

An epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.