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- Mouse Anti-Mouse I-Ek-PE
Product short description
Price:
304 EUR
Size:
100ug
Catalog no.:
GEN673651
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Purification method
N/A
Other names
N/A
Clone
14-4-4S
Clonality
Monoclonal
Latin name
Mus musculus
Category
Secondary Antibodies
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Also known as
Mouse Anti-Mouse I-Ek
Immunoglobulin isotype
Mouse (C3H.SW) IgG2a kappa
Form/Appearance
R - phycoerythrin (R - PE) Conjugate
Tested applications:
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
An epitope on mouse I-Ek MHC class II alloantigen The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes.1 Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen.2 The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D.3; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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