Mouse Anti-Mouse H-2Dd-UNLB

359 EUR

500ug

GEN673688

N/A

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34-5-8S

Monoclonal

Mus musculus

Secondary Antibodies

Mnoclonal antibodies

Mouse (Mus musculus)

Mouse Anti-Mouse H-2Dd

Purified (UNLB) Antibody

Mouse (C3H alpha BDF1) IgG2a kappa

Flow Cytometry; Immunoprecipitation; Immunoaffinity Chromatography

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta 2-microglobulin.2 The antibody does not react with H-2Dd alpha chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H- 2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.5-9; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.