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- Mouse Anti-Porcine CD8a-FITC
Product short description
Price:
547 EUR
Size:
500ug
Catalog no.:
GEN673817
Product detailed description
Purification method
N/A
Concentration
N/A
Also known as
CD8a
Clone
76-2-11
Clonality
Monoclonal
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Category
Secondary Antibodies
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Immunoglobulin isotype
Mouse (BALB/c) IgG2a kappa
Form/Appearance
Fluorescein (FITC) Conjugate
Tested applications:
Flow Cytometry; Immunoprecipitation
Gene name
CD8A; CD8A; CD8; MAL; p32; Leu2; MAL
Gene name synonims
CD8A; CD8A; CD8; MAL; p32; Leu2; MAL
Other gene names
CD8A; CD8A; CD8; MAL; p32; Leu2; MAL
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other names
CD8a molecule; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 alpha chain; T8 T-cell antigen; OTTHUMP00000160760; OTTHUMP00000160764; OTTHUMP00000203528; OTTHUMP00000203721; T cell co-receptor; OKT8 T-cell antigen; T-cell antigen Leu2; Leu2 T-lymphocyte antigen; CD8 antigen, alpha polypeptide (p32); T-lymphocyte differentiation antigen T8/Leu-2; CD8a molecule; T-lymphocyte differentiation antigen T8/Leu-2
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
Porcine CD8a, Mr 32-34 kDa Porcine CD8, a type I transmembrane glycoprotein, is expressed as either a CD8 alpha alpha homodimer or a CD8 alpha beta heterodimer. It is found on most thymocytes, and on the "suppressor/cytotoxic" subpopulation of peripheral T cells. CD8 functions primarily as a co-receptor with MHC Class I-restricted T-cell receptors in antigen recognition. MAb 76-2-11 reacts with the CD8 alpha chain.1-3; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
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