Mouse Anti-Porcine B Cell Subset-PE

365 EUR

100ug

GEN673832

N/A

N/A

N/A

N/A

N/A

N/A

cell

BB6-10A10

Monoclonal

Mus musculus

B Cell Subset

Mouse (Mus musculus)

Mnoclonal antibodies

Secondary Antibodies

Mouse (BALB/c) IgM kappa

R - phycoerythrin (R - PE) Conjugate

Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections)

Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.For cells, cell lines and tissues in culture till half confluency.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Indeterminate MAb BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer's patches (ILPP); 6-17 in spleen; and 0% in thymus.1 While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by BB6-10A10 is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.