Mouse Anti-Chicken TCRgd-FITC

503 EUR

500ug

GEN673876

N/A

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N/A

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TCR1

Monoclonal

Mus musculus

TCR gamma delta

Mouse IgG1 kappa

Anti-FITC Antibody

Mouse (Mus musculus)

Mnoclonal antibodies

Secondary Antibodies

Fluorescein (FITC) Conjugate

Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

Chicken TCR gamma delta MAb TCR1 precipitates a heterodimer of Mr 90-kDa (two bands of Mr 40-kDa and 50-kDa upon reduction) on chicken peripheral blood T cells.1,2 Deglycosylation of the heterodimer yields two polypeptides of Mr 35-kDa and 32-kDa from TCR1 precipitates. Unlike human and mouse, in which gamma delta cells comprise a minor subset of T lymphocytes in the circulation, the chicken has a relatively large subset of gamma delta T cells.1-3 The frequency of TCR gamma delta cells is usually 20-25% of the total blood T cells, but may reach approximately 50% in chickens of 6 months of age.2 The majority of TCR gamma delta + cells in the thymus and blood are CD4-CD8-, although a small subset of them may express CD8 or CD4 coreceptors.3 However, when the TCR gamma delta + cells migrate into the spleen and intestine, most of them begin to express CD8. Although the biological function of gamma delta T cells is unclear, they are clearly capable of cytotoxic activity in vitro. The CD8+ gamma delta T cells may also be involved in down-regulation of the immune response.4 They cannot, however, induce a graft-vs-host (GVH) reactions.5; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.