Mouse Anti-Chicken CD28-FITC

503 EUR

500ug

GEN673893

AV7

N/A

N/A

CD28

Monoclonal

Mus musculus

Mouse IgG1 kappa

Anti-FITC Antibody

Secondary Antibodies

Mnoclonal antibodies

Mouse (Mus musculus)

Cd28; N/A; Cd28; N/A

Cd28; N/A; Cd28; N/A

Cd28; N/A; Cd28; N/A

Fluorescein (FITC) Conjugate

Flow Cytometry; Immunoprecipitation

CD28; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; OTTMUSP00000002157; CD28 antigen; N/A

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added; Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The FITC conjugate is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1% sodium azide as preservative (PBS/NaN3); Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. All products should be stored at the antibodys should be kept in the range of 1-7 degrees Celsius.. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28 Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma delta + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V beta 1- and V beta 2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2 MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.3; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.