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- Mouse Anti-Chicken CD8b-UNLB
Product short description
Price:
387 EUR
Size:
500ug
Catalog no.:
GEN673902
Product detailed description
Purification method
N/A
Concentration
N/A
Clone
EP42
Also known as
CD8 beta
Clonality
Monoclonal
Latin name
Mus musculus
Form/Appearance
Purified Antibody
Immunoglobulin isotype
Mouse IgG2a kappa
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Category
Secondary Antibodies
Other gene names
LOC100136222; N/A; N/A; N/A
Gene name synonims
LOC100136222; N/A; N/A; N/A
Gene name
LOC100136222; N/A; N/A; N/A
Other names
CD8 beta; N/A; CD8 beta; N/A; N/A
Tested applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
beta chain (Mr 34 kDa) of chicken CD8 In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain.1 While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8 alpha - and CD8 beta -chains, a relatively large proportion of the CD8+ TCR- gamma delta cells in the spleens of embryos and young chicks express only the alpha -chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR- gamma delta CD8 alpha beta cells in the chicken that is not found in rodents.3 Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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