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- Mouse Anti-Chicken MHC Class I-PE
Product short description
Price:
365 EUR
Size:
100ug
Catalog no.:
GEN673932
Product detailed description
Purification method
N/A
Concentration
N/A
Clone
F21-2
Clonality
Monoclonal
Also known as
MHC Class I
Latin name
Mus musculus
Immunoglobulin isotype
Mouse IgG1 kappa
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Category
Secondary Antibodies
Other gene names
N/A; gamr-UA-c24; N/A; N/A
Gene name synonims
N/A; gamr-UA-c24; N/A; N/A
Gene name
N/A; gamr-UA-c24; N/A; N/A
Form/Appearance
R - phycoerythrin (R - PE) Conjugate
Other names
MHC class I; MHC class I; N/A; N/A; N/A
Tested applications:
Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot; Immunoaffinity Chromatography
Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
A monomorphic epitope on avian MHC Class I molecules (Mr ~ 68 kDa) Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~ 44 kDa) non-covalently bound to the invariant beta 2-microglobulin subunit (Mr ~ 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3 MAb F21-2 also reacts with turkey MHC Class I.4; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
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