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- Mouse Anti-Human CD14-FITC
Product short description
Price:
326 EUR
Size:
100 Tests
Catalog no.:
GEN674210
Product detailed description
Purification method
N/A
Concentration
N/A
Also known as
CD14
Clone
UCHM-1
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IgG2a
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Category
Secondary Antibodies
Form/Appearance
Fluorescein (FITC) Conjugate
Other gene names
NDUFA2; NDUFA2; B8; CD14; CIB8; N/A
Gene name synonims
NDUFA2; NDUFA2; B8; CD14; CIB8; N/A
Gene name
NDUFA2; NDUFA2; B8; CD14; CIB8; N/A
Tested applications:
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other names
CD14; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; OTTHUMP00000159890; OTTHUMP00000223709; complex I B8 subunit; NADH-ubiquinone oxidoreductase subunit CI-B8; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa; Complex I-B8; CI-B8; NADH-ubiquinone oxidoreductase B8 subunit
Test
MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
Human CD14 CD14 is a 53-55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.1-4 ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
The purified (UNLB) antibody is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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