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- MBS Monoclonals
- Mouse Anti-Human CD74-FITC
Product short description
Price:
304 EUR
Size:
100 Tests
Catalog no.:
GEN674314
Product detailed description
Clone
LN2
Purification method
N/A
Concentration
N/A
Also known as
CD74
Clonality
Monoclonal
Immunoglobulin isotype
Mouse IgG1
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Category
Secondary Antibodies
Form/Appearance
Fluorescein (FITC) Conjugate
Other gene names
CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG
Gene name synonims
CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG
Gene name
CD74; CD74; II; DHLAG; HLADG; Ia-GAMMA; DHLAG
Tested applications:
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
MBS Monoclonals supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
Human CD74 CD74 represents a type II integral membrane protein known as MHC Class II-associated invariant chain (Mr 43, 41, 35, 33 kDa). It is moderately expressed on B cells, and weakly on monocytes and macrophages. CD74 is also found on a subset of activated T cells, Langerhans cells, dendritic cells and some epithelial cells. CD74 may play a role in antigen processing and presentation through intracellular transport and expression of Class II molecules on the cell surface.1-4 ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Other names
CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated), isoform CRA_c; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen gamma chain; p33; HLA-DR-gamma; OTTHUMP00000224283; OTTHUMP00000224284; OTTHUMP00000224285; OTTHUMP00000224286; OTTHUMP00000224288; OTTHUMP00000224338; MHC HLA-DR gamma chain; Ia-associated invariant chain; gamma chain of class II antigens; HLA-DR antigens-associated invariant chain; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33
Storage and shipping
The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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